Palestinians from Gaza launched Freedom 2 in a second attempt to break the Israeli-led siege of the Strip.
The boat set sail for the Cypriot port of Limassol carrying a number of wounded Palestinians and students who have been unable to leave the Gaza Strip.
The Committee for the Great March of Return and Breaking the Siege said the move comes as the Palestinian Authority increases measures of collective punishment against Palestinians in Gaza in a desperate attempt to break the will of those living in the Strip.
It called on all parties to protect the boat from the piracy of the occupation forces, stressing that it will continue its efforts to break the siege and end the inhuman suffering experienced by the residents of the Gaza Strip.
On 29 May, the committee launched the Freedom Ship from Gaza to the world, carrying carrying 25 patients, students and activists, though it travelled further than the Israeli-stipulated fishing limits, the boat was seized and all those on board arrested. Captain Suhail Al-Amoudi was later charged.