Some 150,000 Syrians have fled Daraa heading to Jordan or towards the Israeli occupied Golan Heights in an effort to escape regime bombing on the area, according to the White Helmets civil defence agency.
Jordan has closed its borders to refugees saying it is no longer able to cope with the number of asylum seekers flooding the country.
Refugees carried signs which read "It is a crime against humanity not to open borders" amongst others calling on neighbouring states to help save their lives.
"The safest place is the border with Israel because the regime and Russian airplanes cannot strike the area near the Israeli border," said Waseem Kiwan, a 36-year-old man from the village of Tafas north of Daraa city.
For the last ten days, Daraa has been subject to intense aerial bombardments and ground attacks, with Syrian regime forces capturing the towns of Busra Al-Harir and Nahta.
The United Nations warned on Tuesday that a total of 750,000 lives are in danger in Daraa, and 45,000 people have been displaced.